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KMID : 0366119980260030232
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Korean Journal of Applied Microbiology & Bioengineering
1998 Volume.26 No. 3 p.232 ~ p.236
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Production of β-Mannanase and β-Mannosidase from Sporolactobacillus sp. M201.
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Abstract
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Production of ft-Mannanase and fl-Mannosidase from Sporolactobacillus sp. M201. Park, Won-Sik, Hwa-Young Kim, and Yong-Jin Choi*. Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea - A bacterial strain producing high levels of an extracellular P-mannanase and intracellular fi-mannosidase and a-galactosidase was isolated from soil. The strain isolated was identified as a strain of Sporolactobacillus sp. and designated as Sporolactobacillus sp. M201. Synthesis of 13-mannanase by Sporolactobacillus sp. M201 was induced by sucrose, maltose, or locust bean gum. The highest induction rate was obtained with 2% locust bean gum added to the culture medium as a sole carbon source. On the other hand, induction of P-mannosidase was observed only with locust bean gum. The optimal media for the enzyme production were established as follows: for R-mannanase; 2% locust bean gum, 0.5% peptone, 0.2% KH,PO,, 80 mg/I MgSO,, and 8 mg/1 ZnSO, (pH 6.0), and for f~-mannosidase; 2% locust bean gum, 0.5% yeast extract, 0.2% KHZPO,, 80 mg/l MgSO and 8 mg/I ZnSO, (pH 5.0). The optimal culture temperatures for production of (3-mannanase and Q-mannosidase were found to be 371C and 30r-, respectively. Under the optimal culture conditions, the production of (3-mannanase and (3-mannosidase reached the highest levels of 10.6 units/ml and 1.35 units/ml after 30 h and 24 h cultivation, respectively.
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